Fig. 9
PLGA disassembles preaggregated Aβ1–42 fibers. ThT kinetic assays showing the disassembly of mature Aβ1–42 fibers and the corresponding fluorescence images in the absence and presence of 25 µM PLGA over 72 h incubation at 27 °C (A, D, E), 37 °C (B, F, G) and 40 °C (C, H, I). Note the faster rate of Aβ disaggegation with the rise of temperature. Histograms showing the quantification of fluorescence intensity measured in the absence and presence of 25 µM PLGA over 72 h incubation at 27 °C (J), 37 °C (K) and 40 °C (L) at saturation which did not differ markedly. STEM images showing matured Aβ1–42 fibers in the absence (M–O) and presence of 25 µM PLGA (P–R) after 72 h incubation at 27 °C (M, P), 37 °C (N, Q) and 40 °C (O, R). Note the presence of PLGA nanoparticles (arrows) in conjugation with disassembled Aβ fibers at different temperatures. DLS analysis representing diameter of different aggregated Aβ1–42 populations in the absence and presence of 25 µM PLGA after 72 h incubation at 27 °C (S), 37 °C (T) and 40 °C (U). Note the decreased diameter of aggregated Aβ1–42 populations in the presence of 25 µM PLGA at different temperatures