Fig. 3

In vitro adhesiveness, cellular uptake, cytotoxicity, and ICD of evaluation of PexD. A Confocal microscope images of B16-F10 cells incubated with free DOX and PexD for 0.5 and 2 h, respectively. Scale bar: 10 μm. B Flow cytometry measurement of B16-F10 cells incubated with free DOX and PexD for 0.5 and 2 h. Scale bar: 10 µm. C Fluorescence intensity analyzed by flow cytometry. The data are expressed as mean ± SD (n = 3). D The adhesion of DiR-labeled Pex to B16-F10 cells with confocal laser scanning microscope. CD44 staining (green), DIR-labeled Pex (red) and nucleus (blue). Scale bar (upside): 10 µm. Scale bar (down): 5 µm. E Relative HMGB1 release from B16-F10 cells treated for 24 h with PexD or DOX (3 μg mL⁻¹). The data are expressed as mean ± SD (n = 3). F Relative ATP release from B16-F10 cells treated for 24 h with PexD or DOX (3 μg mL⁻¹). The data are expressed as mean ± SD (n = 3). G Representative flow cytometric analysis of CD80⁺ CD86⁺ cells. The data are expressed as mean ± SD (n = 3). C–F **P < 0.01; ***P < 0.001; ****P < 0.0001