Fig. 5

DPSC derived exosomes can inhibit ROS-MAPK-NFκB P65 signaling pathway in vivo. ROS level was detected by using FACS with a flow cytometry. A and B Mice are analyzed at 3 days after injured spinal cord. C and D Mice are analyzed at 5 days after injured spinal cord. There was a marked increase in ROS levels in the injured spinal cord at 3 days and 5 days. E and F At 3 days, the ERK1/2 and p-ERK1/2 protein expression in spinal tissues was investigated. G and H At 5 days, the ERK1/2 and p-ERK1/2 protein expression in spinal tissues was either investigated. There was also a higher P-ERK/ERK level in the SCI group than in the sham group at 3 days and 5 days. *p < 0.05, ***p < 0.01.