Fig. 4

The biocompatibility of TC-WS-CQDs. A–D Cell viability of Huh7 cells and L02 cells, CCK8 assay (A, B), MTS assay (C, D). Cells were seeded to 96-well plates (8 × 10³ cells/well). E, F Toxicity assessment of TC-WS-CQDs in BALB/nu mice (n = 5). The mice were intravenously injected with TC-WS-CQDs or PBS (300 μl) every 2 days. The blood and organs were collected at day 14, E Complete blood count test and serum biochemistry results. F Histological evaluation of the major organs of the mice. WBC white blood cells, PLT platelet, RBC red blood cells, Hb hemoglobin, TBIL total bilirubin, ALT alanine aminotransferase, ALP alkaline phosphatase, Scr serum creatinine, LDH lactate dehydrogenase. Data represent mean ± SD (n = 3 in A–D, n = 5 in E, F, Mann–Whitney test or unpaired t test were used when appropriate for statistical significance analysis in E. ns: not significant)