Fig. 6
From: Tryptophan-sorbitol based carbon quantum dots for theranostics against hepatocellular carcinoma
ROS generation by photoexcited TC-WS-CQDs. Huh7 cells were incubated with TC-WS-CQDs and exposed to 470 nm irradiation for 10 min. After 24 h, the cellular ROS was tested by DCFH-DA, fluorescence microscope measuring (A), microplate reader detection (B), flow cytometry (C). The data in B–C are mean ± SD (n = 3, statistical significance was analyzed via unpaired t test, *p < 0.05, ***p < 0.001). D ESR spectra of the TC-WS-CQDs in different irradiation time with 470 nm laser. (A–C, CQDs + I470nm: TC-WS-CQDs with 470 nm irradiation and DCFH-DA staining, I470nm: 470 nm irradiation and DCFH-DA staining without TC-WS-CQDs, CQDs: TC-WS-CQDs and DCFH-DA staining without irradiation, Control: DCFH-DA staining without TC-WS-CQDs and irradiation, CQDs-con: adding TC-WS-CQDs only without irradiation and DCFH-DA staining, NC: blank control, no TC-WS-CQDs, irradiation and DCFH-DA staining)