Fig. 2
In vitro evaluation of Raw264.7 cells polarization. a–d ELISA results of TNF-ɑ, IL-4, IL-6, and IL-10 respectively. e Immunofluorescent staining of Raw264.7 cells after cultured for 4 days. f, g Quantitative analysis of iNOS and Arg-1. h The polarization of Raw264.7 cells was analyzed by flow cytometry. i–l RT-qPCR results of Arg-1, IL-10, iNOS, and TNF-ɑ after Raw264.7 cells cultured for 4 days. m Immunofluorescent staining of Notch1. n The expression of Notch1 was detected by western blotting. o The expression of Notch1 protein was quantitatively analyzed by Image J. (n = 3; *, # and + represent P < 0.05 when compared with Ti/SF, Ti/SF/MOF and Ti/SF/I, respectively; **, ## and ++ represent P < 0.01)