Fig. 3
Detection of osteogenic differentiation markers of rBMSCs. a, b ALP staining and activity of rBMSCs cultured in conditioned medium for 7 and 14 days. c ALP immunofluorescent staining of rBMSC in conditioned medium for 7 days: green (ALP), red (actin), blue (Nucleus/DAPI). d OCN immunofluorescent staining of rBMSC in conditioned medium for 14 days: green (ALP), red (actin), blue (Nucleus/DAPI). e Alizarin Red staining of rBMSC cultured in conditioned medium for 14 days. f Quantitative analysis of Alizarin Red staining. g ALP, BMP-2 and OCN western blotting bands of rBMSC in conditioned medium for 14 days. h–j The expressions of ALP, BMP-2 and OCN proteins were quantitatively analyzed by Image J. (n = 3; *, # and + represent P < 0.05 when compared with Ti/SF, Ti/SF/MOF and Ti/SF/I, respectively; **, ## and ++ represent P < 0.01)