Fig. 5

Representative images of 3D ultrasound reconstructions of abdominal aorta, photographs of vessel isolations, immunohistochemistry, and Martius Scarlet Blue demonstrating profound effect of VCAM-1–targeted miR-carrying microbubbles. A 3D ultrasound reconstruction of abdominal aorta shows vessel lumen (in red), as well as massive areas of plaque build-up and aneurysm (in blue), from animals treated with Targ^MB-A₁₂₆ or Targ^MB-S₁₂₆ but not in animals treated with Targ^MB-M_126. B Vessel isolation shows clean abdominal aorta in mice treated with Targ^MB-M₁₂₆ but plaque build-up and aneurysms in mice given Targ^MB-S₁₂₆ or Targ^MB-A₁₂₆. C Immunohistochemistry confirmed a decrease in VCAM-1 expression for Targ^MB-M₁₂₆ treated animals as compared to those treated with Targ^MB-A₁₂₆ or Targ^MB-S₁₂₆. D Martius Scarlet Blue showed plaque build-up and aneurysms in abdominal arteries of Targ^MB-A₁₂₆ or Targ^MB-S₁₂₆ treated animals, whereas very little plaque build-up was observed in Targ^MB-M₁₂₆ treated mice [63]