Fig. 5

miRNA-29a-3p enhanced PD-L1 expression by targeting PTEN expression. A Enhanced expression of PD-L1 at mRNA level in pEXO-educated monocyte (n = 8). B Flow cytometry analysis of the expression of PD-L1 on pEXO-educated monocytes. N = 3, each dot represents one sample. C Increased frequencies of CD14⁺PD-L1⁺ monocytes from pregnant women and non-pregnant control. (Right). N = 11, each dot represents one sample. Non-p: non-pregnant; P: pregnant. D Representative histogram of PD-L1 expression in monocyte after miRNAs transfection. E Quantitative analysis of PD-L1 level in monocyte after miRNAs transfection. F Overlapped target genes between miRNA-10-5p, miRNA-22-3p and miRNA-29-3p by TargetScan 7.2 predication. G Western Blot analysis of PTEN expression after miRNAs mimics transfection. H Quantitative analysis of PTEN expression 24 h after miRNA-29a-3p mimics transfection. I The level of PTEN in monocytes of pregnant women and non-pregnant women (P & Non-P). J Quantitative analysis of PTEN expression of the circulating monocytes of pregnant women and non-pregnant women. (N = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 compared to the control group