Fig. 4
Anticancer efficacy of BATNS in vitro. A Cellular uptake analysis results of the same concentration of DOX labeled BATNS in Hep1-6 cells by flow cytometry analysis (50 μg/mL). B Fluorescence images of Hep1-6 cells treated with DOX labeled BATNS (25 μg/mL) and stained with DAPI (nucleus) for 0, 2, 4, 8 and 12 h, respectively. C The Hep1-6 cells viability of BPNS and BATNS at various concentrations with/without 808 nm NIR laser for 24 h (1 W/cm2, 10 min) (*p < 0.05). D Annexin V-FITC/PI double staining results under different treatment (100 μg/mL, 1 W/cm2, 5 min). E Histogram of apoptosis of Hep 1–6 cells detected by AnnexinV/PI staining matched with D