Fig. 2
Fluorescence quenching ability measurement of Fe3O4@DOP NPs at different concentrations (0, 0.1, 0.2, 0.4, 0.6, 0.8, 1.0 mg/mL) on H1-FAM/H2-FAM before and after HCR amplification. A, B Quenching effect of Fe3O4@DOP NPs (0, 0.1, 0.2, 0.4, 0.6, 0.8 and 1.0 mg/mL) on the fluorescence of H1-FAM (10 nM) and H2-FAM (10 nM). The error bars represent the standard deviations (SD) of three independent experiments (n = 3). C, D HCR amplification evaluation using electrophoresis. The gel was stained with GelRed (C) or detected by the fluorescence of FAM (D). Lanes in both C and D showed the HCR product supplement with T-mimic at the concentrations of 25 nM, 50 nM, or 0 nM) containing 500 nM each H1-FAM and 500 nM H2-FAM. M: DNA marker