Fig. 5
The PGE2 matrix stimulated angiogenesis of the ischemic hindlimbs in vivo. A Representative BLI images revealed the angiogenic tendency of ischemic tissues treated with the PGE2 matrix (n = 3). B Quantitative analysis of the Fluc signal in units of photons/s/cm2/steradian. C Immunohistochemical staining with CD31 on day 14 revealed capillaries in injured muscle sections (n = 5). The bar represents 100 μm. D Quantification of CD31 expression, angiogenesis-related marker in injured muscles. E Immunofluorescence staining with α-SMA on day 28 revealed arterioles at ischemic sites (n = 5). The bar represents 100 μm. F Quantification of immunostaining of angiogenesis-related makers α-SMA immunostaining in injured muscles. Data are expressed as mean ± SD. *P < 0.05 versus the PGE2 group; #P < 0.05 versus the control group. Hindlimb ischemia mice injected with collagen as a control group