Fig. 6
The PGE2 matrix improved the angiogenic effects of HUVECs in vitro. A Representative images of HUVECs treated with the PGE2 matrix for 48 h were stained with Ki-67. The scale bar represents 100 μm. B Images of the wound healing assay in HUVECs treated with PGE2 matrix for 0 and 12 h. The bar represents 200 μm. C The migration ratio of each group. D Images of the tube formation assay of HUVECs treated with the PGE2 matrix for 12 h. The bar represents 200 μm. Quantification of the branch number (E) and node number (F) in three random fields in each group. Data are expressed as mean ± SD. *P < 0.05 versus the PGE2 group; #P < 0.05 versus the control group. HUVECs cultured on a collagen-coated plate as a control group. The experiments were carried out in triplicate