Fig. 7
The PGE2 matrix promoted angiogenesis by decreasing apoptosis of HUVECs in vitro. A Representative images showed apoptosis (cleaved caspase-3, green) of HUVECs. Scale bar, 100 μm. B Quantification of immunostaining of cleaved caspase-3. C Flow cytometry of annexin V and PI. D Quantification of apoptotic cells. E Real-time PCR analysis of HUVEC gene expression related to apoptosis. The HUVECs were cultured in plates coated with PGE2 matrix for 48 h and then treated with 200 μM H2O2 for 6 h. Data are expressed as mean ± SD. *P < 0.05 versus the PGE2 group; #P < 0.05 versus the control group. HUVECs cultured on a collagen-coated plate treated with H2O2 as a control group. The experiments were carried out in triplicate