Fig. 8
The PGE2 matrix improved MyoD1-mediated skeletal muscle regeneration. A Representative images of immunohistochemical staining of MyoD1 in ischemic limbs with different treatment on day 7. B Quantification of MyoD1 immunostaining in injured muscles. C Expression of the MyoD1 gene in ischemic muscle with different treatment on day 7. D Western blot analysis of MyoD1 expression of MyoD1 in injured muscles on day 7. E Representative images of ischemic limbs with different treatment on day 7 stained for Ki-67. The red arrowheads highlighted Ki-67 + muscle cells. Data are expressed as mean ± SD. n = 5. Scale bar, 100 μm. *P < 0.05 versus the PGE2 group; #P < 0.05 versus the control group. Hindlimb ischemia mice injected with collagen as a control group