Fig. 3

In vitro therapeutic evaluation of ITIC-Th NPs. A Fluorescence images of ITIC-Th NPs with a different concentration in PBS (Excitation wavelength: 660 nm). B Relationship between concentration of ITIC-Th and NIR fluorescence intensity. C CCK-8 evaluated the cell cytotoxicity assay. Leuk-1 cells were treated with various formulations for 24 h. D The fluorescence of Live/Dead cells. Live and dead cells were signaled in green and red fluorescence, respectively. Scale bar = 100 μm. E Fluorescence photographs of cellular ROS generation treated with ITIC-Th NPs (10 μg/mL). Scale bar = 100 μm. F Representative immunoblots indicated different protein levels in CAL 27 from each treatment group, and the quantitative analysis was shown in G. H Representative immunoblots indicated different protein levels in Leuk-1 from each treatment group, and the quantitative analysis was shown in I (**p < 0.01; ***p < 0.001)