Fig. 13
MSNs for tissue engineering and regenerative medicine. A Fluorescent images of human ESCs treated with tetramethylrhodamine (TRITC)-loaded MSNs (TMSNs) (i) TRITC and (ii) overlaid with a bright-field image of ESCs. B TMSN-AA nanoconjugates induced the differentiation of human ESCs toward cardiomyocytes (subjected to western blot analysis using antibodies against myocardial marker genes cTnI and FLK-1. Anti-actin was used as a loading control). Reproduced with permission from Ref. [380] Copyright 2015, Elsevier. C–F SEM images showing the attachment and spreading of hMSCs to the membranes after 3 and 24 h: (C1-C2) pure poly(lactide-co-glycolide) (PLGA); (D1-D2) PLGA-10% SBA-15. Cellular morphology and alignment of the hMSCs on different membranes: Cellular actin filaments stained with fluorescein isothiocyanate-phalloidin (green) and nuclei counterstained with 4′,6′-diamidino-2-phenylindole (blue) E pure PLGA; F PLGA-10% SBA-15. Reproduce with permission from Ref. [386] Copyright 2014, American Chemical Society