Fig. 3
From: A drug-free nanozyme for mitigating oxidative stress and inflammatory bowel disease
CeNP-PEG exhibited high antioxidant activities against multiple ROS. ESR spectra showing concentration-dependent scavenging activities of CeNP-PEG to ROS, including •OH (A), O2•− (B) and H2O2 (C). D Intracellular ROS levels in BMDMs was determined by flow cytometry after the treatment with complete medium, 100 ng/mL LPS + 20 ng/mL IFN-γ in the presence or absence of CeNP-PEG (0.5 or 1.0 μg/mL). E Representative fluorescence microscopy images of BMDMs isolated from mice after the indicated treatments. Scale bar = 100 μm. F A proposed schematic illustration of the antioxidant mechanism of CeNP-PEG against multiple ROS via reversible valence state switching between Ce3+ and Ce4+. BMDMs: Bone marrow-derived macrophages. LPS: Lipopolysaccharide. DCF: 2’, 7’-Dichlorodihydrofluorescein