Fig. 5

BHB inhibits the differentiation of osteoclast. A Representative TRAP staining of osteoclast. Scale bar: 200 μm. B Quantification of multi-nucleated TRAP-positive cells. C Representative image of TRAF6, NFATc-1, c-Fos after invention with BHB by western blot. D–F Quantification of TRAF6, NFATc-1, and c-Fos. n = 3. G The gene expression of TRAF6. n = 3. H Representative co-staining (NFATc-1 and TRAF2) immunofluorescence image of osteoclast. Scale bar: 100 μm. I–J The gene expression of NFATc-1 and c-Fos. Results are mean ± SEM. *p < .05