Fig. 2
A In vitro viability of 4T1 cells and HUVEC incubated with Fe2O3@TA-Pt NPs for 24 or 48 h. B The endocytosis of Fe2O3@TA-Pt NPs. Scale bars: 10 μm. C The production of γ-H2A.X in 4T1 cells treated with different treatments (blue: DAPI, green: phalloidin, red: γ-H2A.X). Scale bars: 10 μm. D Platinum internalized in 4T1 cells (red) and corresponding Pt-DNA adducts (blue) after incubation with Fe2O3@TA-Pt NPs at different times (20 and 120 min). n = 3, ***p < 0.001. E In vitro viability of 4T1 cells treated with RT differed in dosage. n = 3, ***p < 0.001. F CLSM images of 4T1 cells subjected to different treatments, and ROS/hypoxia detection probes were used as indicators. Scale bars: 10 μm. G 4T1 cells in G2/M phase of cell cycle upon different treatments