Fig. 4
A In vivo fluorescence imaging of mice received intravenous injection of Cy5.5-labeled Fe2O3@TA-Pt. These images were collected at 1, 2, 4, 8, 12, and 24 h post-injection. B The fluorescence images of organs harvested at 72 h post-injection. C In vivo biodistribution of Pt at different time periods (1, 2, 4, 8, 12 and 24 h). D PET images of mice with different treatments (the red circles indicated tumors, different colors represented different levels of [18 F]MISO uptake), the PET was conducted 1 h after the injection of [18 F]MISO. E Immunofluorescence images showing the distribution of CD31-positive blood vessels (green) and nuclei (blue) in tumor tissue at different time points. Scale bar: 100 μm. F Standardized uptake values (SUVs) of [18 F]MISO in tumor obtained from PET images (left panel) and relative blood vessel area as shown in E (right panel). n = 3, ***p < 0.001