Fig. 5

Cur@SF NPs reduce oxidative stress and restore the mitochondrial function of the cerebellum in YG8R mice. All samples were from tissue cerebellum. A Detection of the catalase and SOD2 protein levels. B–D The enzymatic activities of SOD, catalase and MDA content in the cerebellum after Cur@SF NP treatment (n = 5). E TEM results of cerebellar tissue to observe mitochondrial morphology. F H&E staining to evaluate the recovery of morphology and array of Purkinje neurons as indicated by the red arrows. G Immunofluorescent staining of NeuN to determine the number of neurons. H Detection of mitochondrial aconitase activity (purple) and mitochondrial protein levels. mAco2: mitochondrial aconitase (Aco2) activity (purple); Ndufs1, SDHB, and Uqcrfs1: mitochondrial complex I/II/III subunits. I The activities of mitochondrial complexes I and II. J ATP content. (*p < 0.05; NS no significance; YG8R + NPs vs. YG8R, n = 5/group)