Fig. 4

Cytotoxicity in HTR-8/SVneo cells induced by CeO₂NPs. A Deposition of CeO₂NPs in HTR-8/SVneo cells observed by TEM (Upper scale bar = 2 μm, lower scale bar = 500 nm). HTR-8/SVneo cells treated with 0, 4, and 8 μg ml⁻¹ CeO₂NPs for 24 h. CeO₂NPs deposited in the cytoplasm (Red arrow). N: nucleus. B Cell viability was evaluated through the CCK-8 assay in HTR-8/SVneo cells upon exposure to CeO₂NPs at different concentrations for 24 h. C Light microscope images with 200 magnification of CeO₂NPs treated cells for 24 h. *P < 0.05 compared with the control group