Fig. 5

Cell culture on composite hydrogel films. a Schematic diagrams of PC12 cells cultured on PS inverse opal films and composite hydrogel films. b PC12 cells cultured on PS inverse opal films: (i) immunofluorescence image, (ii) SEM image, (iii) angle distribution of PC12 cells. c PC12 cells cultured on composite hydrogel films: (i) immunofluorescence image, (ii) SEM image, (iii) angle distribution of PC12 cells. d Length of neurites along the stretching direction changes with culture days. e Length of neurites perpendicular to the stretching direction changes with culture days. All these substrates were stretched at 30°. Scale bars are 50 μm in all (i) and (ii)