Fig. 4
From: Quantitative hypoxia mapping using a self-calibrated activatable nanoprobe
Quantitative hypoxia imaging in vitro. a Fluorescence images of hypoxic and normoxic 4T1 cells incubated with Cy7-1/PG5-Cy5@LWHA; blue: DAPI staining of nucleus, green: “always-on” Cy5 fluorescence of the nanoprobe, red: activatable Cy7-1 fluorescence of the nanoprobe. b Pseudo-color ICy7-1/ICy5 ratio images of hypoxic and normoxic 4T1 cells incubated with Cy7-1/PG5-Cy5@LWHA. c Quantitative analysis of ICy7-1/ICy5 ratio in hypoxic and normoxic 4T1 cells, error bars, mean \(\pm\) s.d. (n = 3), p < 0.0001