Fig. 3

Luminescent properties and tissue penetration ability of LAD NPs. A Dose-dependent luminescent intensities of LAD NPs at 80 mM H₂O₂. B Effects of H₂O₂ levels on luminescent signals of 20 mg/mL LAD NPs. C MPO-dependent luminescence profiles of 20 mg/mL LAD NPs at 5 mM H₂O₂. In all cases, the left panels show luminescence images, while the right panels indicate quantitative data. D, E Typical luminescent images (D) and corresponding quantitative data of the same concentration of LAD NPs and LCD NPs at 80 mM H₂O₂ in the presence of emission filters of different wavelengths. F, G Luminescence images (F) and quantitative results (G) of the same concentration of LAD NPs and LCD NPs (at 15 mg/mL) in a black 96-well plate covered by 1 or 2 layers of 3-mm pork ham slices upon incubation with 80 mM H₂O₂. H Quantified luminescence intensities of LCD NPs or LAD NPs at 80 mM H₂O₂ and in the presence of different thicknesses of ham slices. Data are presented means ± SD (n = 3). **P < 0.01