Fig. 6
ExosomeYoung promoted migration and FMT by delivering miR-125b. A DiI-labeled exosomes were engulfed by fibroblasts as shown. Scale bar: 20 μm. B MiR-125b mRNA in fibroblastOld was examined using qRT-PCR. *P < 0.05 versus Control; #P < 0.05 versus exosomeYoung+miR−125b inhibitor in repeated measures analysis of variance (n = 6). C Images of migrated fibroblastsOld using Transwell migration assays. D Data are presented as the number of migrated cells. E, F The mRNA levels of the pro-fibrotic genes α-SMA and Col1 were analyzed using qRT-PCR. G Quantification of α-SMA-positive cells. H Representative images of immunofluorescence staining of α-SMA. I FACS plots detailing the gating strategy to define SMA-positive subpopulations. J Quantification of the relative abundance of SMA-positive cells. *P < 0.05 versus Control; #P < 0.05 versus exosomeYoung+miR−125b inhibitor in repeated measures analysis of variance (n = 6)