Fig. 9

The design of Engineered-Exo. A Representative images showing the live and dead ADSCs (Parental ADSCs, agomir-23a-3p ADSCs and agomir-nc ADSCs) within porous Gelma hydrogel, and the rate of live cell. Scale bar: 500 μm. Green: live ADSCs. Red: dead ADSCs. B Quantitative RT-PCR analysis showed the level of hsa-mir-23a-3p within three types of ADSCs at 1 day and 10 day after transfection. C Protein markers of Engineered-Exo, Exo 4 and their corresponding ADSCs extract quantified by western blotting. D Quantitative RT-PCR analysis showed the level of hsa-mir-23a-3p within microtia chondrocytes at 24 and 48 h post-treatment. E Western blotting analysis was used for quantifying the protein levels of PTEN, P-PI3K/PI3K, P-AKT/ AKT and mTOR in microtia chondrocytes at 72 h post-treatment. F Quantitative RT-PCR analysis showed the mRNA level of COL2A1, ACAN, ELN, SOX9 and COL1A1 within microtia chondrocytes at 48 h post-treatment. Data was presented as the mean ± SD of three number of replicates. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, ns: no significance