Fig. 2

Anti-inflammatory effect of F3-derived EVs on LPS-treated BV2 cells and 6-OHDA-treated SH-SY5Y cells. A A heatmap of cytokine and chemokine levels in each group was shown. The changed levels were indicated in colors (yellow-increased, Purple-decreased). (Group: DPBS, LPS 0.01 µg/mL treated, 25 µg of HFF-EV treated with LPS, 25 µg of F3-EV treated with LPS). B Five significantly decreased integrated intensity of each cytokine and chemokines in the F3 EV + LPS group compared to LPS and HFF EV + LPS groups were identified. C Schematic illustration of the experimental design to validate the change of pro-inflammatory cytokine in BV2 (microglia cell line) co-cultured with 6-OHDA-treated SH-SY5Y cells using the transwell system. D, E After 24 h of treatment with F3-derived EVs to BV2 (bottom well), the expression of IL-1α and IL-1β of BV2 cells were measured using qPCR. Intracellular IL-1α and IL-1β levels were increased in BV2 cells cultured with 6-OHDA-treated SH-SY5Y cells in transwell, but the expression of these cytokines was not increased by F3-derived EVs