Fig. 4

Preliminary toxicity study in vivo and in vitro. A MTT assays of CD90@NP and T-CD90@NP in primary chondrocytes; n = 3. B Flow cytometry assays of annexin V and propidium iodide (PI) in T-CD90@NP and IL-1βtreated primary chondrocytes; n = 3. C–J ELISA analysis of alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine (CREA), blood urea nitrogen (BUN), white blood cell (WBC) count, red blood cell (RBC) count, hemoglobin (HGB) and platelet (PLT) levels in different groups; n = 3. **P < 0.01, ***P < 0.001, N = no statistical differences. K H&E staining of the heart, liver, spleen, lung and renal tissue in the different groups; Bar = 100 μm, n = 3