Table 1 qPCR primers for key factors of rat chondrocyte cycle
Name | Gene name | Forward (5′ → 3′) | Reverse (5′ → 3′) |
|---|---|---|---|
Gapdh | GAPDH | GCATCTTCTTGTGCAGTGCC | GATGGTGATGGGTTTCCCGT |
Cyclin D1 | CCND2 | ACCTGTGAGGAAGCCATTCG | CCAGCGTGTCCCTTCTCATT |
Cyclin D3 | CCND3 | ACACGCGTCGCTTCTCCTA | TGTGACATCTGTGGGAGTGC |
CDK4 | CDK4 | ACCAGGATCTCCCACTAGCA | TCAGGTCCCGGTGAACAATG |
CDK6 | CDK6 | GGCCGCAGTAGTCAGTTACC | CCAACACTCCAGAGGTCCAC |
Cyclin E2 | CCNE2 | CGCAGTAGCCGTTTACAAGC | TCACTGCAAGCACCATCAGT |
Cyclin A2 | CCNA2 | GTCAACCCCGAAAAAGTGGC | GCCTTCCATGTGTCTGACCAA |
CDK2 | CDK2 | AGGCGGCAACATTGTTTCAA | GACAGGGACTCCAAAGGCTC |
Cyclin B1 | CCNB1 | GGTCGATGTGGAGCAGCATA | GGCAAAATGCACCATGTCGT |
Cyclin B2 | CCNB2 | TGGCTGGTCCAAGTCCATTC | TGTGCTGCATGACTTCCAGT |
CDK1 | CDK1 | AGGACCAGCTCACAAAAGGG | GTGGAAAAGCGGCTTCTTGG |
P16 | CDKN2A | AACACTTTCGGTCGTACCCC | CTCCCTCCCTCTGCTAACCT |
P21 | CDKN1A | AAGCCCGAGTTCCTGCTAAC | ATCGGCGCTTGGAGTGATAG |
P27 | CDKN1B | GACTCACTCGCGGCTCC | TGTTTACGTCTGGCGTCGAA |