Fig. 4
From: Bacteria-driven hypoxia targeting delivery of chemotherapeutic drug proving outcome of breast cancer
In vitro invasiveness and cytotoxicity of various treatments on 4T1 cells. A Wound healing. B The healing rate of Control, DOX, DOX-NPs, Bif and Bif@DOX-NPs at 6, 12 and 24 h. C Flow cytometry analysis of apoptosis rates of 4T1 cells induced by different preparation groups. D Schematic illustration of the hypoxia simulation model using a Transwell system to evaluate the chemotaxis of Bif@DOX-NPs. E Number of bacteria migrating to the bottom chamber. F Bif@DOX-NPs and hypoxic zone co-localization in vivo. Hypoxic zone was stained green with 488@HIF-1α (Anaerobic induction factors labelled with Alexa Fluor 488), and Bif was stained red with Cy3@Ab (Bifidobacterium infantis antibodies labelled with Cy3). (G) Fluorescence co-localization analysis of Bif@DOX-NPs and hypoxic zone. Results are expressed as mean ± SD (n = 3, ns no statistical significance, *P < 0.05, ***P < 0.001, ****P < 0.0001)