Fig. 4
Effect of a targeted anti-cancer probe on human hepatic carcinoma cells. A Different probes were used (Dulbecco’s modified Eagle high-glucose medium (DMEM), glucose oxidase (GOD), Fe3O4 nanoparticles (NPs), Fe3O4-GOD NPs (FG NPs)) to stimulate tumor cells to produce H2O2 in neutral (pH = 7.4) media. B Average OCR (pmoles/min) of Hep-G2 cells incubate with DMEM (pH = 7.4, n = 3) and DMEM (pH = 6.5, n = 3). C Average OCR (pmoles/min) of Hep-G2 cells incubate with 6.25 µg/ml Fe3O4 NPs (pH = 7.4, n = 3). D Average OCR (pmoles/min) of Hep-G2 cells incubate with different concentrations (0.39 μg/ml, 1.56 μg/ml, 6.25 μg/ml) of FG NPs. E Fe3O4 NPs and FG NPs stimulate tumor cells to produce ROS in acidic (pH = 6.5) and neutral media (pH = 7.4). F The viability of Hep-G2 cells. G Live-dead cell staining kit and flow cytometry analysis of each probe (Fe3O4 NPs and FG NPs) at different concentrations in acidic (pH = 6.5) and neutral media (pH = 7.4). **P < 0.01; ***P < 0.001, ns not significant