Fig. 2
Screening the DP-AD by delivery efficiency and antisense efficacy in vitro. a FAM-positive ratio of ESBLs-E. coli (left) and MRSA (right) were tested by flow cytometry after incubation with FAM-labeled DP-AD for 1 h in dark at 37 °C. Free FAM-labeled ASOs (red) and LF2000-NPs (light blue) were used as negative and positive controls, respectively. b GFP fluorescence intensity of E. coli (DH5α) expressing the GFP measured by flow-cytometry after incubation with 1 μM DP-ADanti-egfp for 3 h. c Growth curves of ESBLs-E. coli treated with different amphipathic DP-ADanti-acpP (1 μM), OD600nm, the optical density at 600 nm