Fig. 3

The effects of HPL-supplemented hydrogel on the characteristics of SHED include proliferation, stemness, osteogenic and adipogenic differentiation. A Cell proliferation of SHED were performed after cultured on HPL-supplemented hydrogel for 24 h. The ordinate represents the relative cell viability; B Alizarin Red and Oil Red O were used for staining to characterize the osteogenic differentiation and adipogenic differentiation of SHED cultured on HPL-supplemented hydrogel. Magnification: 4 × 10; C Expression of the mRNA of the cell stemness gene NANOG; D The mRNA expression of osteogenic differentiation markers ALP of stem cells; E qPCR was used to detect the expression of osteogenic differentiation marker genes RUNX2; F The mRNA expression of osteogenic differentiation markers OSTEOPONTIN of stem cells; G qPCR was used to detect the expression of adipogenic differentiation genes LPL; H The expression of adipogenic differentiation marker genes PPARγ2 was detected by RT-qPCR; ALP alkaline phosphatase, RUNX2 runt-related transcription factor 2, PPARγ peroxisome proliferator activated receptor gamma, LPL lipoprotein lipase. The data was shown in mean ± SEM. ***p < 0.001, **p < 0.01, *p < 0.05, ns: no significant difference. Three independent experiment repetitions were performed