Fig. 5

Confirmation of the specificity of the immunohistochemistry of GPC3_3 aptaprobe using cancer cell lines. A Fluorescence microscopy images of fluorescein amidite (FAM)–GPC3_3 aptaprobe binding to GPC3-expressing cells. FAM–GPC3_3 aptaprobe population (green) has been incubated with different cell lines (top to down: HepG2, Hep3B, Hela cells, PANC-1, and MIA-PaCa). Nuclear counter stain was used as a control (blue 4′,6-diamidino-2-phenylindole [DAPI] staining). The cells were treated with 0, 50, 100, and 200 pM FAM-modified GPC3_3 aptaprobe. Both DAPI and FAM fluorescence are only shown in HepG2 and Hep3B cells. B The flow cytometry results for evaluating GPC3 aptaprobe binding specificity using 200 pM FAM-modified GPC3_3 aptaprobe and the five different cells used for flow cytometry. The flow cytometry results of cells not treated with aptamer (red) and cells treated with aptamer (green) were expressed by overlapping in one diagram. As with the confocal imaging results, the FAM signal appeared only in HepG2 and Hep3B cells