Fig. 5
Effects of PTC-M on inflammation, apoptosis and MAPK pathway in H2O2 treated HK-2 cell. A Comparison of expression level for HO-1 proteins determined by immunoblotting in HK-2 after stimulation with vehicles or H2O2. β-actin was used as the endogenous control. B Images of the effects of H2O2-induced ROS production by PTC-M imaged by fluorescence microscope. Cytosolic ROS was labeled using DCF-DA probes. C Comparison of expression level for apoptosis and MAPK proteins determined by immunoblotting in HK-2 after stimulation with vehicles or H2O2. D Flow cytometry results with Annexin V-FITC/PI staining. Apoptotic cells were defined as Annexin V+ and PI−. Data are shown as mean ± SEM. *P < 0.05 vs. control (CON) group; #P < 0.05 vs. H2O2. group; †P < 0.05 vs. PTC-M group. HO-1 heme oxygenase-1, DCF-DA 2′,7′-dichlorodihydrofluorescein diacetate, MAPK mitogen-activated protein kinase