Fig. 8

Effects of PTC-M on inflammation in IRI mice kidneys. A Comparison of protein expression level for HO-1 determined by immunoblotting from the kidney of sham, IRI, and IRI + PTC-M mice. β-actin was used as the endogenous control. B Comparison of mRNA expression level for inflammatory markers determined by qPCR from the kidney of sham, IRI, and IRI + PTC-M mice. C Representative images of immunohistochemical staining for HO-1 (left) and F4/80 (right) in the kidney of sham, IRI and IRI + PTC-M mice. Scale bars, 25 μm. Data are shown as mean ± SEM. *P < 0.05 vs. sham mice; ^#P < 0.05 vs. IRI mice. MCP-1 monocyte chemoattractant protein-1, TNF-α tumor necrosis factor-α, ICAM-1 intercellular adhesion molecule-1, VCAM-1 vascular cell adhesion molecule-1, TGF-β transforming growth factor beta