Fig. 4

Silencing Nrf2 reversed the impaired RANKL-induced osteoclastogenesis. A Representative TRAP staining images and quantification of TRAP-positive cells transfected with both siNrf2 and siNOX4. Scale bars, 100 µm. B The expression of osteoclast-specific genes (C) and osteoclast-specific proteins. D Representative fluorescence images of F-actin rings. Scale bars, 50 µm. E Representative SEM images after cells were transfected with both siNrf2 and siNOX4. Scale bars, 200 µm. *p < 0.05, **p < 0.01