Fig. 4
From: Monitoring in vivo behavior of size-dependent fluorescent particles as a model fine dust
Flow cytometric and microscopic analysis of single-cells obtained from the excised lungs. a Gating strategy to identify epithelial and endothelial cells and leukocytes. b Qualitative analysis of cellular uptake of CSPM0.1 in lung cells. Populations of CSPM0.1-labeled c stromal cells (CD45−) and leukocytes (CD45+) and d epithelial (CD45−CD326+) and endothelial cells (CD45−CD31+). e Qualitative analysis of cellular uptake of CSPM2.0 in lung cells. Populations of CSPM2.0 labeled f stromal cells (CD45−) and leukocytes (CD45+) and g epithelial (CD45−CD326+) and endothelial cells (CD45−CD31+). h Cellular microscopy images of CSPM-labeled cells. Red, green, and blue colors represent CSPMs, CD45 antibodies, and DAPI staining, respectively (scale bar = 10 µm)