Fig. 6

EXO-Hep reduced the mitochondria mediated damage to OGD-stimulated SH-SY5Y cells by promoting transfer of healthy mitochondria of astrocytes into neurons. A Schematic diagram of co-incubation of neurons with culture medium of astrocyte and purified astrocytic mitochondria. B The cell viability of OGD-stimulated SH-SY5Y cells incubating with CM/AS, CM/A1-AS, CM/A1-AS (EXO), CM/A1-AS (Hep) and CM/A1-AS (EXO-Hep). Data represent means ± SD (n = 3), ***P < 0.001. C The cell viability of OGD-stimulated SH-SY5Y cells incubating with Mito/AS, Mito/A1-AS, Mito/A1-AS (EXO), Mito/A1-AS (Hep) and Mito/A1-AS (EXO-Hep). Data represent means ± SD (n = 3),*P < 0.05, **P < 0.01, ***P < 0.001. D Images and quantitative analysis of apoptotic OGD-stimulated SH-SY5Y cells incubating with culture medium and purified mitochondria from A1 astrocytes treated with Hep, EXO and EXO-Hep in TUNEL assay. The scale bar is 100 μm and applies to all Figure D, Data represent means ± SD (n = 3), **P < 0.01, ***P < 0.001. E Western blot analysis on the expressions of associated apoptotic proteins such as BAX and BCL-2 in OGD-stimulated SH-SY5Y cells treated with Mito/AS, Mito/A1-AS, Mito/A1-AS (EXO), Mito/A1-AS (Hep) and Mito/A1-AS (EXO-Hep). Data represent means ± SD (n = 3),*P < 0.05, **P < 0.01, ***P < 0.001