Fig. 10

Myocardium-targeted NO release effectively relieved LPS-induced myocardial pro-inflammatory response. a–d Inflammatory cytokines including IL-1, IL-6, IL-18, and TNF-α in serum measured 6 h after different treatments (n = 8). Data were presented as Mean ± SD. *P < 0.05; **P < 0.01. e The expression of inflammatory mediator proteins NLRP3 and NF-κB P65. f, g Quantitative analysis of NLRP3 and NF-κB P65 expression (n = 8). Data were presented as Mean ± SD. *P < 0.05; **P < 0.01. h The percentage of Gr-1⁺ cells in the heart tissue (n = 8). Data were presented as Mean ± SD. *P < 0.05; **P < 0.01. i Immunohistochemical analysis of cardiac tissue slices with Gr-1⁺ staining. DAPI (blue; nuclei); Gr-1⁺ positive (brown; black arrow) (scale bar = 100 μm)