Fig. 1

Characterization of RGD-PLT@PLGA-FE. A Schematic diagram of the preparation of RGD-PLT@PLGA-FE. B Representative TEM image of RGD-PLT@PLGA-FE. Scale Bar = 100 nm. Red circles showed PLT membranes that coated at the surface of PLGA nanoparticles. C The hydrodynamic diameters and Zeta potentials of the PLT membrane vesicles, PLGA-FE and RGD-PLT@PLGA-FE. n = 3/group. D The stability of RGD-PLT@PLGA-FE in water, PBS and 50% FBS. n = 3/group. E Release curve of FE from RGD-PLT@PLGA-FE before and after the additional wash. n = 3/group. F Protein composition of platelet vesicle (PLT-ghost), PLT@PLGA, and RGD-PLT@PLGA analyzed by Coomassie staining. G. CD62p and CD41 expression in PLT-ghost, PLT@PLGA, and RGD-PLT@PLGA determined by Western blot. n = 3/group. H Quantitative analysis of expression of CD62p in PLT-ghost, PLT@PLGA and RGD-PLT@PLGA. n = 3/group. I Quantitative analysis of expression of CD41 in PLT-ghost, PLT@PLGA and RGD-PLT@PLGA. n = 3/group. J Dot blot analysis of extracellular immunoglobulin domain of CD47 in PLGA only, PLT@PLGA, RGD-PLT@PLGA. K Quantitative analysis of extracellular immunoglobulin domain of CD47 in PLGA, PLT@PLGA and RGD-PLT@PLGA. n = 3/group. Data presented as mean ± SD. ***p < 0.001