Fig. 5

Immunohistochemical analysis of angiogenesis and neurogenesis of stroke mice. A Experimental scheme. B The pattern diagram of the peri-infarct region. Fluorescence imaging and quantitative analysis were performed in C1, C2, C3 and E. C Pictures showed CD31⁺ (green) microvessels in the peri-infarct region at 14 days after stroke. Scale Bar = 100 μm. D Pictures showed CD31⁺ (red) /Ki67⁺(green) cells in the peri-infarct region at 14 days after stroke. Scale Bar = 50 μm. E Pictures showed DCX⁺ (green) cells in the SVZ at 14 days after stroke. Scale Bar = 75 μm. F Quantitative analysis of the number of blood vessels of mice that treated with 10% sucrose, FE, PLGA-FE, PLT@PLGA-FE, RGD-PLT@PLGA, RGD-PLT@PLGA-FE at 14 days after stroke. n = 4, 4, 3, 4, 4, 4 (from left to right). G Quantitative analysis of the number of CD31⁺/Ki67⁺ cells of mice that treated with 10% sucrose, FE, PLGA-FE, PLT@PLGA-FE, RGD-PLT@PLGA, RGD-PLT@PLGA-FE at 14 days after stroke. n = 3/group. H Quantitative analysis of DCX⁺ cells in SVZ of mice that treated with 10% sucrose, FE, PLGA-FE, PLT@PLGA-FE, RGD-PLT@PLGA, RGD-PLT@PLGA-FE at 14 days after stroke. n = 4, 3, 3, 4, 4, 4 (from left to right). Data presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001