Fig. 6

Visualization of CSFV or SARS-CoV-2 probe entering cells using live-cell fluorescence analysis. A Confocal microscopy analysis of CSFV probe into PAM cells infecting by CSFV. Visualization of CSFV or SARS-CoV-2 probe entering cells using live-cell fluorescence analysis. A Confocal microscopy analysis of CSFV probe into PAM cells infecting by CSFV. LV-NC + PAM is the mock infection group to CSFV probe into PAM cells. The PAM are the negative control experiments without CSFV infection. The probe was marked with FAM with green color and the cellular nucleus was stained blue (DAPI). The merged image clearly revealed the co-incubation of CSFV probe with PAM cells. B Confocal microscopy analysis of SARS-CoV-2 probe into RAW264.7 cells transfecting by plasmid including SARS-CoV-2 N gene. The pN1-NC + RAW is as mock infection to SARS-CoV-2 probe into RAW cells by pN1-NC transfection. The RAW are the negative control experiments without the pEOFP-N1-N plasmid transfection containing the SARS-CoV-2 N gene. The probe was marked with FAM with green color and the cellular nucleus was stained blue (DAPI). The merged image clearly revealed the co-incubation of SARS-CoV-2 probe with RAW264.7 cells. CSFV, classical swine fever virus; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2; PAM, porcine macrophage cells; RAW, RAW264.7 cells