Table 1 PCR primer sequences
From: Modulated nanowire scaffold for highly efficient differentiation of mesenchymal stem cells
Gene | Primer Sequence (5′–3′) | Length (bp) |
|---|---|---|
GAPDH (housekeeping) | F: ATGGGGAAGGTGAAGGTCG R: TAAAAGCAGCCCTGGTGACC | 70 |
ALP | F: GGAACTCCTGACCCTTGACC R: TCCTGTTCAGCTCGTACTGC | 86 |
Runx2 (Cbfa1) | F: TGGCAGTCACATGGCAGATTTC R: TGCTAAATTCTGCTTGGGTGGG | 148 |
Osteocalcin | F: GGCAGCGAGGTAGTGAAGAG R: CTCACACACCTCCCTCCTG | 102 |
Osteopontin | F: CAAACGCCGACCAAGGAAAA R: GGAGGCAAAAGCAAATCACTGC | 60 |