Fig. 2

Comparison effect of SEVs from CHDFs and LWDFs in vitro and in vivo. A mRNA levels of COL1A1 and WB images of collagen I and fibronectin in CHDFs treated with CH/LW-SEVs. n = 3. B Collagen I content of CHDFs treated with CH/LW-SEVs. n = 3. C Proliferation of CHDFs treated with CH/LW-SEVs by CCK-8. n = 3. D EdU analysis of CHDFs treated with CH/LW-SEVs. n = 3. Scare bars = 100 μm. E Cell cycle of CHDFs treated with CH-SEVs and LW-SEVs. Data are the percentage of G1, G2/M, and S. n = 3. F Wound recovery area of CHDFs treated with CH-SEVs and LW-SEVs. Objective area is between the two red line. n = 3. G Images of collagen gel contraction in CHDFs treated with CH/LW-SEVs. n = 3. H Location of CH-SEVs in mouse skin by IVIS imaging system (on the left) and frozen section (on the right). The 100 µL of SEVs labelled with DiR dye were injected by subcutaneous in the buttocks of nude mice. The supematant of free SEVs-labelled was used as a negative control (NC). n = 3. I Skin thickness of mice treated with CH/LW-SEVs. n = 3. J Collagen I content of mouse skin treated with CH/LW-SEVs. n = 3. Data was calculated using student’s t test and two-way ANOVA followed by Bonferroni’s multiple comparisons test. Data are expressed as means ± SEM; with P* < 0.05, P** < 0.005, P*** < 0.001