Fig. 2
Comparison of performance of the developed SERS with the agglomeration-free system. A SERS spectra of different control groups and B the corresponding SERS intensity at 1340 cm−1. (a) MBP, (b) MBP + AuNP-hp1, (c)MBP + AuNP-hp1 + AuNP-hp2, (d)MBP + AuNP-hp1 + miRNA, and (e) MBP + AuNP-hp1 + AuNP-hp2 + miRNA; the concentration of miRNA-499 used was 1 pM. C Representative SERS spectra collected by the present platform in the presence of miRNA-499 at various concentrations. D A standard curve constructed based on the intensity of the 1340 cm−1 peak obtained from miRNA-499 detection by the present platform. E Representative SERS spectra collected by the agglomeration-free system in the presence of miRNA-499 at various concentrations. F Linear regression based upon the intensity of the 1340 cm−1 peak obtained from miRNA-499 detection by the agglomeration-free system