Fig. 1
Physiochemical and biological characterization of the RCM-fibers. A Representative fluorescent images of the RCM-fibers after coumarin (for PLGA nanofiber) and Dil (for lipid bilayer) staining. Scale bar = 20 μm. B Detection of Na+/K+ ATPase and CD11c proteins on the RCM-fibers (with LPS/IFN-γ activated RAW264.7 cell membrane) and Un-fibers (without cell membrane) by Western blotting. C SDS-PAGE analysis of protein profiles of LPS/IFN- γ activated RAW264.7 cell membrane (RCM) and the RCM-fibers. D Representative images of the RCM-fibers morphology by the scanning electron microscopy. Scale bar = 10 μm (left) or 5 μm (right). E Size distribution of the RCM-fibers. F Comparisons of water contact angels between the Un-fibers and the RCM-fibers. G Comparisons of degradation rates between the Un-fibers and the RCM-fibers. H Fluorescent images of BMMSCs cultured on the Un-fibers or the RCM-fibers by CFDA-SE staining. Scale bar = 50 μm. I CCK-8 assays on cell viability of BMMSCs at day 1, day 3, day 5 and day 7 with the presence of H2O2. Data were represented as mean ± SD. Differences were assessed by using one-way ANOVA with Tukey’s multiple comparison tests. *P < 0.05, **P < 0.01