Fig. 5
RCM-fibers promote the expressions of wound healing related genes in BMMSCs facilitating keratinocyte migration and less inflammation. A Schematic illustration showing the cocultures of BMMSCs with the RCM-fibers in vitro. B Gene ontology enrichment scatter plots in key signaling pathways. C Heat map analysis of wound healing related gene expressions in BMMSCs after coculturing with either the Un-fibers or the RCM-fibers for 48 h. D Validation of expression levels of CD200, Ccl2, Lif, TNFAIP6 and Lcn2 in BMMSCs after coculturing with either the Un-fibers or the RCM-fibers for 48 h by real-time PCR. E Schematic illustration on the migration of JB6 cell incubated with either the RCM-fiber-BMMSCs or the Un-fiber-BMMSCs upon H2O2 treatment. F Representative images of the wound areas covered by JB6 cell at 12, 18, 24 and 36 h. Scale bar = 50 μm. G Comparisons of normalized wound areas covered by JB6 cell at different time points. H CD86 and CD206 expressions on LPS/IFN-γ activated RAW264.7 cells after incubating with the RCM-fiber-BMMSCs or the Un-fiber-BMMSCs. I TNF-α expression in LPS/IFN-γ activated RAW264.7 cells after incubating with the RCM-fiber-BMMSCs or the Un-fiber-BMMSCs. Data were represented as mean ± SD. Differences were analyzed by one-way ANOVA with Tukey’s multiple comparison test. The experiments were repeated in triplicates. *P < 0.05, **P < 0.01